CGM - Département Dynamique et Stabilité des Génomes
Ségrégation des chromosomes et division cellulaire
Responsable : François-Xavier BARRE
MàJ : 10/01/11
Composition de l'équipe
François-Xavier Barre, Directeur de Rech. (2)
Ariane David, Doctorante (5)
Gaëlle Demarre, Postdoctorante (6)
Eriel Martinez, Postdoctorant
Caroline Midonet, Etudiante Master 2
Evelyne Paly, Assistante-Ingénieur
Christophe Possoz, Chargé de Rech. CNRS (1)
Adresse
CNRS - Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 26
91198 GIF-SUR-YVETTE Cedex
FRANCE
Tél. : 33 (0)1 69 82 32 24
Fax : 33 (0)1 69 82 31 60
Financements
Le groupe bénéficie d’un financement de 3 ans du programme “EMBO Young Investigator” de 2006, d’un financement de 3 ans du programme “Excellence” de la FRM de 2007 et d’un financement de 4 ans du programme “ANR Blanc” de 2009.
Domaine d'activité
Lors de la prolifération, la synthèse de l’ADN, la ségrégation des chromosomes et la division cellulaire doivent être coordonnées pour assurer le maintien de l’information génétique. La séparation temporelle de ces processus et l’existence de points de contrôle assurent cette coordination chez les eucaryotes. Chez les bactéries, il n’y a pas de séparation temporelle. Au contraire, ces processus sont interdépendants. Chez Escherichia coli, par exemple, la formation du septum de division sur des chromosomes partiellement ségrégés est nécessaire à la résolution des dimères de chromosome (1, 2). Ainsi, la synthèse de l’ADN, la ségrégation des chromosomes et la division cellulaire forment un système intégré.
Le groupe explore les mécanismes moléculaires sous-jacents à cette intégration dans deux organismes modèles : E. coli, qui porte un unique chromosome circulaire, et Vibrio cholerae, qui porte deux chromosomes circulaires (3).
Parallèlement, nous étudions le mode d’intégration de CTXf, un vibriophage qui pirate le système de résolution des dimères de chromosomes de son hôte pour s’intégrer. L'un des déterminants majeurs de la pathogénicité de V. cholerae, la toxine cholérique, est codé dans le génome de CTXf. Son intégration est un élément clef dans l’émergence de nouvelles souches pathogènes de vibrios (4, 5). Comprendre le mode d’intégration de CTXf est donc important en terme d’épidémiologie et de risque environnemental.
Références :
1. Dubarry, N. and Barre, F.-X. (2010) Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. EMBO J, 29 (3) 597-605.
2. Kennedy, S.-P., Chevalier, F. and Barre, F.-X. (2008) Delayed activation of Xer recombination at dif by FtsK during septum assembly in Escherichia coli. Mol Microbiol, 68 (4) 1018-28.8.
3. Val, M.-E., Kennedy, S.-P., El Karoui, M., Bonné, L., Chevalier, F. and Barre, F.-X. (2008) FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae. PLoS Genet, 4 (9) e1000201.
4. Das, B., Bischerour, J., Val, M.-E. and Barre, F.-X. (2010) Molecular keys of the tropism of integration of the cholera toxin phage. Proc Natl Acad Sci U S A, 107 (9) 4377-82. doi: 10.1073/pnas.0910212107
5. Val, M., Bouvier, M., Campos, J., Sherratt, D., Cornet, F., Mazel, D., and Barre, F. (2005). The single-stranded genome of phage CTX is the form used for integration into the genome of Vibrio cholerae. Mol Cell 19, 559-566.
Mots-clefs :
Recombinaison spécifique de site, ségrégation des chromosomes, division cellulaire, lysogénie, Escherichia coli, Vibrio choleare
Groupes travaillant sur des sujets proches :
Dr J.-F. Allemand
Dr F. Cornet
Dr F. Boccard
Dr Didier Mazel
Dr B. Michel
Pr David J. Sherratt
Publications (depuis 1998)
Possoz, C., Junier, I., Espeli, O. (2012) Bacterial chromosome segregation. Front Biosci, 17, 1020-34. Review.
Das, B., Bischerour, J., Barre, FX. (2011) Molecular mechanism of acquisition of the cholera toxin genes. Indian J Med Res, 133 (2) 195-200.
Das, B., Bischerour, J. Barre, F. X. (2011) VGJ{phi} integration and excision mechanisms contribute to the genetic diversity of Vibrio cholerae epidemic strains. Proc Natl Acad Sci U S A, 108 (6) 2516-21.
Das, B., Bischerour, J., Val, M.-E. and Barre, F.-X. (2010) Molecular keys of the tropism of integration of the cholera toxin phage. Proc Natl Acad Sci U S A, 107 (9) 4377-82.
Manosas, M., Meglio, A., Spiering, MM., Ding, F., Benkovic, SJ., Barre, FX., Saleh, OA., Allemand, JF., Bensimon, D., Croquette, V. (2010) Magnetic tweezers for the study of DNA tracking motors. Methods Enzymol, 475, 297-320. Walter, NG. Ed., Academic Press, San Diego, USA.
Dubarry, N., Possoz, C., Barre, F-X. (2010) Multiple regions along the Escherichia coli FtsK protein are implicated in cell division. Mol Microbiol, 78 (5) 1088-100.
Dubarry, N. and Barre, F.-X. (2010) Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. EMBO J, 29 (3) 597-605.
Bonné, L., Bigot, S., Chevalier, F., Allemand, J.-F. and Barre, F.-X. (2009) Asymmetric DNA requirements in Xer recombination activation by FtsK. Nucleic Acids Res, 23 (7) 2371-80.
Val, M.-E., Kennedy, S.-P., El Karoui, M., Bonné, L., Chevalier, F. and Barre, F.-X. (2008) FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae. PLoS Genet, 4 (9) e1000201.
Kennedy, S.-P., Chevalier, F. and Barre, F.-X. (2008) Delayed activation of Xer recombination at dif by FtsK during septum assembly in Escherichia coli. Mol Microbiol, 68 (4) 1018-28.
Barre, F.-X. (2007) FtsK and SpoIIIE: the tale of the conserved tails. Mol Microbiol. 66 (5) 1051-1055. Review.
Bigot, S., Sivanathan, V., Possoz, C., Barre, F.-X. and Cornet, F. (2007) FtsK, a literate chromosome segregation machine. Mol Microbiol, 64 (6) 1434-41. Review.
Bigot, S., Saleh, O. A., Cornet, F., Allemand, J.-F. and Barre, F.-X. (2006) Oriented loading of FtsK on KOPS. Nat Struct Mol Biol, 13 (11) 1026-8.
Lionnet, T., Dawid, A., Bigot, S., Barre, F.-X., Saleh, O., Heslot, F., Allemand, J.-F., Bensimon, D. and Croquette, V. (2006) DNA mechanics as a tool to probe helicase and translocase activity. Nucleic Acids Res, 34 (15) 4232-44.
Barre, F.-X. and Sherratt, D. J. (2005) Chromosome dimer resolution. In The bacterial chromosome N. P. Higgins (Ed.), ASM press.
Val, M., Bouvier, M., Campos, J., Sherratt, D., Cornet, F., Mazel, D., and Barre, F. (2005). The single-stranded genome of phage CTX is the form used for integration into the genome of Vibrio cholerae. Mol Cell 19, 559-566.
Saleh, O., Bigot, S., Barre, F., and Allemand, J. (2005). Analysis of DNA supercoil induction by FtsK indicates translocation without groove-tracking. Nat Struct Mol Biol 12, 436-440.
Lesterlin, C., Mercier, R., Boccard, F., Barre, F.-X., and Cornet, F. (2005). Roles for replichores and macrodomains in segregation of the Escherichia coli chromosome. EMBO Rep 6, 557-562.
Bigot, S., Saleh, O., Lesterlin, C., Pages, C., El Karoui, M., Dennis, C., Grigoriev, M., Allemand, J., Barre, F., and Cornet, F. (2005). KOPS: DNA motifs that control E. coli chromosome segregation by orienting the FtsK translocase. EMBO J 24 3770-3780.
Barre, F.-X., and Sherratt, D. J. (2005). Chromosome dimer resolution. In The bacterial chromosome, P. N. Higgins, ed. (Washington, D. C., ASM press), pp. 513-524.
Bigot, S., Corre, J., Louarn, J., Cornet, F., and Barre, F. X. (2004). FtsK activities in Xer recombination, DNA mobilization and cell division involve overlapping and separate domains of the protein. Mol Microbiol 54, 876-886.
Lesterlin, C., Barre, F. X., and Cornet, F. (2004). Genetic recombination and the cell cycle: what we learned from chromosome dimers. Mol Microbiol 54, 1151-1160.
Massey, T. H., Aussel, L., Barre, F.-X., and Sherratt, D. J. (2004). Asymmetric activation of Xer site-specific recombination by FtsK. EMBO Rep 5, 399-404.
Saleh, O. A., Perals, C., Barre, F. X., and Allemand, J. F. (2004). Fast, DNA-sequence independent translocation by FtsK in a single-molecule experiment. EMBO J 23, 2430-2439.
Lau, I. F., Filipe, S. R., Soballe, B., OA, O., Barre, F. X., and Sherratt, D. J. (2003). Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol Microbiol 49, 731-743.
Ip, S. C., Bregu, M., Barre, F. X., and Sherratt, D. J. (2003). Decatenation of DNA circles by FtsK-dependent Xer site-specific recombination. EMBO J 22, 6399-6407.
Yates, J., Aroyo, M., Sherratt, D. J., and Barre, F. X. (2003). Species specificity in the activation of Xer recombination at dif by FtsK. Mol Microbiol 49, 241-249.
Barre, F.-X., and Sherratt, D. J. S. (2002). Xer Site-Specific Recombination: Promoting Chromosome Segregation. In Mobile DNA II, N. L. Craig, R. Craigie, M. Gellert, and A. Lambowitz, eds. (Washington, D.C., ASM Press), pp. 149-161.
Aussel, L., Barre, F. X., Aroyo, M., Stasiak, A., Stasiak, A. Z., and Sherratt, D. (2002). FtsK is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases. Cell 108, 195-205.
Sherratt, D. J., Lau, I. F., and Barre, F. X. (2001). Chromosome segregation. Curr Opin Microbiol 4, 653-659.
Sherratt, D. J., Soballe, B., Barre, F. X., Filipe, S., Lau, I., Massey, T., and Yates, J. (2004). Recombination and chromosome segregation. Philos Trans R Soc Lond B Biol Sci 359, 61-69.
Barre, F. X., Soballe, B., Michel, B., Aroyo, M., Robertson, M., and Sherratt, D. (2001). Circles: The replication-recombination-chromosome segregation connection. Proc Natl Acad Sci U S A 98, 8189-8195.
Barre, F. X., Ait-Si-Ali, S., Giovannangeli, C., Luis, R., Robin, P., Pritchard, L. L., Helene, C., and Harel-Bellan, A. (2000). Unambiguous demonstration of triple-helix-directed gene modification. Proc Natl Acad Sci U S A 97, 3084-3088.
Barre, F. X., Aroyo, M., Colloms, S. D., Helfrich, A., Cornet, F., and Sherratt, D. J. (2000). FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev 14, 2976-2988.
Barre, F. X., Asseline, U., and Harel-Bellan, A. (1999). Asymmetric recognition of psoralen interstrand crosslinks by the nucleotide excision repair and the error-prone repair pathways. J Mol Biol 286, 1379-1387.
Barre, F. X., Giovannangeli, C., Helene, C., and Harel-Bellan, A. (1999). Covalent crosslinks introduced via a triple helix-forming oligonucleotide coupled to psoralen are inefficiently repaired. Nucleic Acids Res 27, 743-749.
Barre, F. X., Mir, L. M., Lecluse, Y., and Harel-Bellan, A. (1998). Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation. Biotechniques 25, 294-296.
Ait-Si-Ali, S., Ramirez, S., Barre, F. X., Dkhissi, F., Magnaghi-Jaulin, L., Girault, J. A., Robin, P., Knibiehler, M., Pritchard, L. L., Ducommun, B., et al. (1998). Histone acetyltransferase activity of CBP is controlled by cycle-dependent kinases and oncoprotein E1A. Nature 396, 184-186.
