CGM - Department Regulation and Compartmentalization of Cellular Functions
Senescence and mitochondria in Podospora anserina
Group leader: Annie SAINSARD-CHANET
Last update: 30-Jun-2011
The team
Elodie Bovier, PhD student (university granted)
Annie Sainsard-Chanet, Professor, Paris-Sud 11
Carole H. Sellem, Researcher, CNRS
Address
CNRS
Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 26
91198 GIF-SUR-YVETTE Cedex
FRANCE
Phone: 33 (0)1 69 82 43 70 - 31 90
Telecopy: 33 (0)1 69 82 31 60
Research activities
Aging research has seen a tremendous boost over the past few years. It has yielded substantial amounts of data on aging in the different model systems that are being used. A comparison between the different model systems shows that mechanisms of aging must have been at least partially conserved in the course of evolution.
Our group uses the filamentous fungus Podospora anserina as a simple model to study aging. This ascomycetous fungus undergoes a distinctive aging process, which is systematically associated with mtDNA instability. We have recently shown that respiratory metabolism has a key role in this process.
Our work aims (1) to deepen our understanding of the link between mitochondria and aging, (2) to understand the mechanisms behind the instability of the mitochondrial chromosome, and (3) to characterize new functions and parameters implicated in life span regulation.
The group is one of the twelve partners that are involved in MiMage - The Role of Mitochondria in Conserved Mechanisms of Ageing - , a project within the 6th Framework Program of the European Commission. This international project aims to characterize evolutionary conserved mitochondrial functions in aging.
Key words
Aging, ageing, senescence, longevity, life span, mitochondria, mtDNA stability, respiratory metabolism, alternative oxidase, free radicals, reactive oxygen species .
Selected publications (since 1999)
Review
Lorin, S., Dufour, A. and Sainsard, A. (2006) Mitochondrial metabolism and aging in the filamentous fungus Podospora anserina. BBA 1757 (5-6) 604-610
Research articles
Déquard-Chablat, M., Sellem, CH., Golik, P., Bidard, F., Martos, A., Bietenhader, M., di Rago, JP., Sainsard-Chanet, A., Hermann-Le Denmat S., Contamine, V. (2011) Two nuclear life-cycle-regulated genes encode interchangeable sub,units c of mitochondrial ATP synthase in Podospora anserina. Mol Biol Evol, 28 (7) 2063-75.
Bénit, P., El-Khoury, R., Schiff, M., Sainsard-Chanet, A., Rustin, P. (2010) Genetic background influences mitochondrial function: modeling mitochondrial disease for therapeutic development. Trends Mol Med, 16 (5) 210-7.
Maas, MF., Sellem, CH., Krause, F., Dencher, NA., Sainsard-Chanet, A. (2010) Molecular gene therapy: Overexpression of the alternative NADH dehydrogenase NDI1 restores overall physiology in a fungal model of respiratory complex I deficiency. J Mol Biol, 399 (1) 31-40.
El-Khoury, R. and Sainsard-Chanet, A. (2010) Deletion of the mitochondrial NADH kinase increases mitochondrial DNA stability and life span in the filamentous fungus Podospora anserina. Exp Gerontol, 45 (7-8) 543-9.
van Diepeningen, A.-D., Maas, M.-F., Huberts, D.-H., Goedbloed, D.-J., Engelmoer, D.-J., Slakhorst, S.-M., Koopmanschap, A.-B., Krause, F., Dencher, N.-A., Sellem, C.-H., Sainsard-Chanet, A., Hoekstra, R.-F. and Debets, A.-J. (2010) Calorie restriction causes healthy life span extension in the filamentous fungus Podospora anserina. Mech Ageing Dev, 131 (1) 60-8.
Maas, M. F., Krause, F., Dencher, N. A. and Sainsard-Chanet, A. (2009) Respiratory Complexes III and IV Are Not Essential for the Assembly/Stability of Complex I in Fungi. J Mol Biol, 387 (2) 259-69.
El-Khoury, R. and Sainsard-Chanet, A. (2009) Suppression of Mitochondrial DNA Instability of adPEO-associated ANT1 Mutations in Podospora anserina. Genetics, 183 (3) 861-71.
Sellem, C., Bovier, E., Lorin, S. and Sainsard-Chanet, A. (2009) Mutations in Two Zinc Cluster Proteins Activate Alternative Respiratory and Gluconeogenic Pathways and Restore Senescence in Long-lived Respiratory Mutants of Podospora anserina. Genetics, 182 (1) 69-78.
Maas, M. F., Krause, F., Dencher, N. A. and Sainsard-Chanet, A. (2009) Respiratory Complexes III and IV Are Not Essential for the Assembly/Stability of Complex I in Fungi. J Mol Biol,387 (2) 259-69.
Boivin, A., Gaumer, S. and Sainsard-Chanet, A. (2008) Life span extension by dietary restriction is reduced but not abolished by loss of both SIR2 and HST2 in Podospora anserina. Mech Ageing Dev 129 (12) 714-721.
Espagne, E., Lespinet, O., Malagnac, F., Da Silva, C., Jaillon, O., Porcel, B., Couloux, A., Aury, J., Ségurens, B., Poulain, J., Anthouard, V., Grossetete, S., Khalili, H., Coppin, E., Déquard-Chablat, M., Picard, M., Contamine, V., Arnaise, S., Bourdais, A., Berteaux-Lecellier , V., Gautheret, D., de Vries, R., Battaglia, E., Coutinho, P., Danchin, E., Henrissat, B., El Khoury, R., Sainsard-Chanet, A., Boivin, A., Pinan-Lucarré, B., Sellem, C., Debuchy, R., Wincker, P., Weissenbach, J. and Silar, P. (2008) The genome sequence of the model ascomycete fungus Podospora anserina. Genome Biol, 9 (5) R77.
El-Khoury, R., Sellem, C.-H., Coppin, E., Boivin, A., Maas, M.-F., Debuchy, R. and Sainsard-Chanet, A. (2008) Gene deletion and allelic replacement in the filamentous fungus Podospora anserina. Curr Genet, 53 (4) 249-58.
Laquel-Robert, P., Sellem, C., Sainsard-Chanet, A. and Castroviejo, M. (2007) Identification and biochemical analysis of a mitochondrial endonuclease of Podospora anserina related to curved-DNA binding proteins. Biochim Biophys Acta-General Subjects, 1770 (4) 527-42.
Maas, M.F., Sellem, C.H., Hoekstra, R.F., Debets, A..J and Sainsard-Chanet, A. (2007) Integration of a pAL2-1 homologous mitochondrial plasmid associated with life span extension in Podospora anserina. Fungal Genet Biol. 44 (7) 659-71.
Sellem, C. H., Marsy, S., Boivin, A., Lemaire, C. and Sainsard-Chanet, A. (2007) A mutation in the gene encoding cytochrome c1 leads to a decreased ROS content and to a long-lived phenotype in the filamentous fungus Podospora anserina. Fungal Genet Biol. 44 (7) 648-58.
Sellem, C., Lemaire, C., Lorin, S., Dujardin, G., and Sainsard-Chanet, A. (2005). Interaction between the oxa1 and rmp1 genes modulates respiratory complex assembly and lifespan in Podospora anserina. Genetics 169, 1379-1389
Lorin, S., Dufour, E., Boulay, J., Begel, O., Marsy, S., and Sainsard-Chanet, A. (2001) Overexpression of the alternative oxidase restores senescence and fertility in a long lived-respiration-deficient mutant of Podospora anserina. Mol Microb 42 : 1259-1267
Bundle of asci - Picture by H. Dalstra & S. Saupe
Dufour, E., Boulay, J., Rincheval, V. and Sainsard-Chanet, A. (2000). A causal link between respiration and senescence in Podospora anserina. Proc. Natl. Acad. Sci. 97 : 4138-4143
Begel, O., Boulay, J. Albert, B., Dufour, E. and Sainsard-Chanet, A. (1999) Mitochondrial group II introns, cytochrome c oxidase and senescence in Podospora anserina. Mol. Cell. Biol. 19: 4093-4100.
Further information
Publications, collaborations, studients ;
For further information on the biology and genetics of Podospora anserina ;
