CGM - Department Genes Expression
Gene Regulation in Salmonella and its Phages
Group leader: Lionello BOSSI
Last update: 21-Feb-2012
Our team
Lionello Bossi, Group Leader, CNRS
Nara Figueroa-Bossi, Research Associate, CNRS
Qi Yang, PhD student, China Scholarship Council
Our address
CNRS - Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 24
91198 GIF-SUR-YVETTE Cedex
FRANCE
Phone : 33 (0)1 69 82 38 11
Telecopy : 33 (0)1 69 82 38 77
Research themes
The research activity our group focuses on three areas, using the bacteria Salmonella enterica (serovar Typhimurium) as a model system:
1. The mechanisms of regulation by small non-coding RNAs in bacteria: to know more about this theme
2. The role of temperate bacteriophage in horizontal gene transfer and the evolution of bacterial genomes: to know more about this theme
3. The role of DNA gyrase in the biological activity of DNA: to know more about this theme
Model system: the bacterium Salmonella enterica (serovar Typhimurium)
Recent publications
Rochat T, Bouloc P, Yang Q, Bossi L, Figueroa-Bossi N. (2012) Lack of interchangeability of Hfq-like proteins. Biochimie, Epub ahead of print.
Lemire, S., Figueroa-Bossi, N., Bossi, L. (2011) Bacteriophage Crosstalk: Coordination of Prophage Induction by Trans-Acting Antirepressors. PLoS Genet, 7 (6) e1002149.
Balbontín, R., Fiorini, F., Figueroa-Bossi, N., Casadesús, J. and Bossi, L. (2010). Recognition of heptameric seed sequence underlies multi-target regulation by RybB small RNA in Salmonella enterica. Molecular Microbiology, 78 (2) 380-94.
Effantin, G., Figueroa-Bossi, N., Schoehn, G., Bossi, L., Conway, JF. (2010) The tripartite capsid gene of Salmonella phage Gifsy-2 yields a capsid assembly pathway engaging features from HK97 and lambda. Virology, 402 (2) 355-65.
Figueroa-Bossi, N., Valentini, M., Malleret, L., Fiorini, F., Bossi, L. (2009) Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target. Genes Dev, 23 (17) 2004-15. Erratum in: Genes Dev. 2010 Apr 1; 24 (7) 734.
Lemire, S., Figueroa-Bossi, N. and Bossi, L. (2008) A singular case of prophage complementation in the mutational activation of recET orthologs in Salmonella enterica serovar Typhimurium. J Bacteriol, 190 (20) 6857-66.
Bossi, L., Maloriol, D. and Figueroa-Bossi, N. (2008) Porin biogenesis activates the sE response in Salmonella hfq mutants. Biochimie, 90 (10) 1539-44.
Balbontín, R., Figueroa-Bossi, N., Casadesús, J. and Bossi, L. (2008) Insertion hotspot for horizontally acquired DNA within a bidirectional small RNA locus in Salmonella enterica. J Bacteriol, 190 (11) 4075-8.
Bossi, L. and Figueroa-Bossi, N. (2007) A small RNA downregulates LamB maltoporin in Salmonella. Mol Microbiol, 65 (3) 799–810.
Lemire, S. (2006) Les prophages de Salmonella typhimurium : régulation lysogénique et contribution à la pathogénicité. Thèse, Paris-Sud 11.
Figueroa-Bossi, N., Lemire, S., Maloriol, D., Balbontin, R., Casadesus, J. and Bossi, L. (2006) Loss of Hfq activates the sigmaE-dependent envelope stress response in Salmonella enterica. Mol Microbiol, 62 (3) 838-52.
Figueroa-Bossi, N., Ammendola, S. and Bossi, L. (2006) Differences in gene expression and in enzyme properties account for the uneven contribution of superoxide dismutases SodCI and SodCII to pathogenicity in Salmonella enterica. Microbes Infect, 8 (6) 1569-1578.
Alonso, A., Pucciarelli, M., Figueroa-Bossi, N., and and Garcia del Portillo, F. (2005). Increased excision of the Salmonella defective prophage ST64B caused by a deficiency in Dam methylase. J Bacteriol, 187 (23) 7901-11. ,
Blanc-Potard, A., Labesse, G., Figueroa-Bossi, N., and Bossi, L. (2005). Mutation at the "exit gate" of the Salmonella gyrase a subunit suppresses a defect in the gyrase B subunit. J Bacteriol, 187, 6841-6844.
Bossi, L., and Figueroa-Bossi, N. (2005). Prophage arsenal of Salmonella enterica serovar Typhimurium. In Phages: Their Role in Bacterial Pathognenesis and Biotechnology, M. Waldor, D. Friedman, and S. Adhya, eds. (Washington, DC, ASM Press).
Coombes, B., Wickham, M., Brown, N., Lemire, S., Bossi, L., Hsiao, W., Brinkman, F., and Finlay, B. (2005). Genetic and molecular analysis of GogB, a phage-encoded type III-secreted substrate in Salmonella enterica serovar typhimurium with autonomous expression from its associated phage. J Mol Biol, 348, 817-830.
Bacciu, D., Falchi, G., Spazziani, A., Bossi, L., Marogna, G., Leori, G., Rubino, S., and Uzzau, S. (2004). Transposition of the heat-stable toxin astA gene into a gifsy-2-related prophage of Salmonella enterica serovar Abortusovis. J Bacteriol, 186, 4568-4574.
Figueroa-Bossi, N., and Bossi, L. (2004). Resuscitation of a defective prophage in Salmonella cocultures. J Bacteriol, 186, 4038-4041.
Bossi, L., Fuentes, J., Mora, G., and Figueroa-Bossi, N. (2003). Prophage contribution to bacterial population dynamics. J Bacteriol, 185, 6467-6471.
Ho, T., Figueroa-Bossi, N., Wang, M., Uzzau S, Bossi, L., and Slauch, J. (2002). Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar Typhimurium. J Bacteriol, 184, 5234-5239.
Uzzau, S., Bossi, L., and Figueroa-Bossi, N. (2002). Differential accumulation of Salmonella [Cu, Zn] superoxide dismutases SodCI and SodCII in intracellular bacteria: correlation with their relative contribution to pathogenicity. Mol Microbiol, 46, 147-156.
Gari, E., Bossi, L., and Figueroa-Bossi, N. (2001). Growth-dependent DNA breakage and cell death in a gyrase mutant of Salmonella. Genetics, 159, 1405-1414.
Uzzau, S., Figueroa-Bossi, N., Rubino, S., and Bossi, L. (2001). Epitope tagging of chromosomal enes in Salmonella. Proc Natl Acad Sci USA, 98, 15264-15269.
Figueroa-Bossi, N., Uzzau, S., Maloriol, D. and Bossi L. (2001) Variable assortment of prophages provides a transferable repertoire of pathogenic determinants in Salmonella. Mol Microbiol, 39, 260-272.
